Biolayer interferometry (BLI) is a label free biomolecular detection method created by Gator Bio co-founder, Hong Tan. In biolayer interferometry, biomolecular interactions are detected by measuring the interference pattern of white light reflected from the surface of a biosensor.

Gator Bio biosensors combine a 1mm diameter glass rod with patented optical layers and specialized surface chemistry built at the distal end of the biosensor. Biolayer interferometry compares the interference pattern of white light reflected from an internal reference layer within a layer of immobilized biomolecules on the surface chemistry of the biosensor. Shifts in interference due to the accumulation of biomolecules are monitored in real-time to sensitively analyze and calculate rates of association and dissociation among target proteins with high precision. Only the interactions of bound biomolecules are measured, making biolayer interferometry a powerful tool for a wide range of applications.